Translational_Unit

Part:BBa_K773004:Design

Designed by: Katie Knister   Group: iGEM12_Caltech   (2012-10-01)

mCherry-AAV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We eliminated a restriction site from the gene. The construct was shown to successfully produce and degrade the fluorescent mCherry protein. To view the characterization assay, go to the [http://2012.igem.org/Team:Caltech/Parts/Characterization Caltech Characterization] page.


Source

This part was modified from a plasmid provided by the Murray lab at Caltech. The people who worked on this part are Ashley Su, Nate Glasser, Emzo de los Santos, Meg Knister, Daisy Lin, and Katie Knister.

References

[http://www.cds.caltech.edu/~murray/wiki/Main_Page The Murray Lab]